<aside> ✍️ Reference Article: https://heyadesai80.medium.com/simulating-e-coli-with-tinkercell-872baf2f8ea4 Youtube video: https://youtu.be/z-pLpeLjB00 Google drive: https://www.youtube.com/redirect?event=video_description&redir_token=QUFFLUhqbGRFUm9haDU4VWxHdFpIUzk0WDhFUlBKNDRGQXxBQ3Jtc0ttQ1N1TVF4VHc0a0pRWU1SSTc1Tk1YdW15Ym10d2IwU0VHSlpVRjFfUFpXazVVQWhKeHg5c1pPUUJILVotaC1hUHNscERteVdKNFdwNHhCWlBWcjBPSFFMclVYeXJVdlVPOXpKNXFDcE1UcldBSHRudw&q=https%3A%2F%2Fdrive.google.com%2Fdrive%2Ffolders%2F15fThz81znyku0wa0JMOTvCHg9g8XuHXf%3Fusp%3Dsharing

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A Bistable System

• The final value of lactose as well as the LacZ enzyme in the system is dependent on how much there is at the beginning
• Amount of lactose stays low when there is a low quantity at the beginning; glucose is the preferred source of fuel

• Uptake is upregulated when there is an adequate quantity in the beginning

Biological Parts

• Promoter: DNA sequences where the process of transcription of a gene by RNA polymerase initiates.
• Repressor Binding Site: Involved in repression — inhibiting or decreasing gene expression.
• Ribosome Binding Sequence: Messenger RNA sequence in mRNA that ribosomes can bind to and subsequently begin translation (protein synthesis)
• Protein Coding Sequence: Encodes amino acid sequence for the protein of interest such as an enzyme or repressor

Processes